FLUORESCENT SENSING POTENTIAL OF SYNTHESIZED 2′,7′-DICHLOROFLUORESCEIN HYDRAZIDE DERIVATIVES: DESIGN, SYNTHESIS, AND CHARACTERIZATION
Keywords:
2′,7′-Dichlorofluorescein, Hydrazides, Aryl Hydrazines, Fluorescent Sensors, Organic DerivativesAbstract
Optical sensors have become valuable alternatives to traditional sensing systems, offering flexibility for a broad range of analytical applications. Among these, small-molecule fluorescent probes have gained considerable interest due to their ability to detect various environmental and biological analytes, combined with advantages such as simplicity, affordability, and rapid response. Nevertheless, most currently available fluorescent probes are selective for only a single analyte, highlighting the need to design new multifunctional fluorescent compounds.
In this study, novel 2′,7′-dichlorofluorescein-based hydrazide derivatives were synthesized using different substituted aryl hydrazines. The reaction progress was monitored through thin-layer chromatography (TLC), while the successful synthesis and structural characterization of the hydrazides were confirmed by Fourier Transform Infrared (FTIR) spectroscopy and proton/carbon nuclear magnetic resonance (¹H/¹³C NMR) spectroscopy. These newly synthesized hydrazide derivatives exhibit strong potential as multifunctional fluorescent sensing materials for a variety of analytical and environmental applications.
